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Western blot test for hiv
Western blot test for hiv











western blot test for hiv

Liu LL, Liu BL, Xie JC, He LM (2016) Analysis of HIV-1 indeterminate Western blot results in Hechuan District, 20. Kline RL, McNairn D, Holodniy M, Mole L, Margolis D, Blattner W, Quinn TC (1996) Evaluation of Chiron HIV-1/HIV-2 recombinant immunoblot assay. Kakaiya R, Gordon S, Zimmerman A, Verlinsky R, Ahmed S, Phillips M (2011) False-positive nucleic acid test results for human immunodeficiency virus RNA and hepatitis C virus RNA: an underappreciated problem. Jamjoom GA, Maatouk J, Gazal M, Damanhouri L, Awliaa A, Ruwaihi N, Bawazeer M, Halabi H, Adel AA, Abdulla A (1997) Follow-up of HIV western blot indeterminate results. Jackson JB, Hanson MR, Johnson GM, Spahlinger TG, Polesky HF, Bowman RJ (1995) Long-term follow-up of blood donors with indeterminate human immunodeficiency virus type 1 results on Western blot. Han Y, Li X, Wei ZG (2016) Analysis of indeterminate results of HIV antibodies in Xianyang City from 2010 to 2015. Guan GY, Wu ZL, Cao M, Yin T, Yang DZ, Chen H (2016) Analysis of HIV Western blot indeterminate results during 2010–2015 in Ningxia. Granade TC, Parekh BS, Tih PM, Welty T, Welty E, Bulterys M, Ndikintum G, Nkuoh G, Tancho S (2005) Evaluation of rapid prenatal human immunodeficiency virus testing in rural cameroon. Gao M, Wu SL, Yan YS (2016) Review of the influence factors and detection methods regarding indeterminate result of HIV antibodies by WB test. Nature 453:289–290įettig J, Swaminathan M, Murrill CS, Kaplan JE (2014) Global epidemiology of HIV. Transfus Med Rev 14:151–160ĭou HF, Zhang HC, Chen D, Zhu CX, Wu MM, Zheng WL (2015) Investigation and analysis of the indeterminate samples of HIV antibody. Braz J Infect Dis 9:506–509ĭodd RY, Stramer SL (2000) Indeterminate results in blood donor testing: what you don’t know can hurt you. J Med Virol 50:293–302Ĭremonezi D, Mesquita PE, Romão MM, Prestes-Carneiro LE (2005) Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente, Brazil. Lab Med Clin 10:659–660Ĭoste J, Montes B, Reynes J, Peeters M, Segarra C, Vendrell JP, Delaporte E, Segondy M (1996) Comparative evaluation of three assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma. MMWR Morb Mortal Wkly Rep 63:537Ĭhu CX, Zhao SP, Liu FF (2013) Analysis on follow-up outcomes of 158 samples of indeterminate HIV antibody. Rev Inst Med Trop Sao Paulo 41:155–158ĬDC (2014) National HIV Testing Day and new testing recommendations. Nucleic acid testing facilitates the identification of HIV infections.Ĭarneiro-Proietti AB, Cunha IW, Souza MM, Oliveira DR, Mesquita NM, Andrade CA, Catalan-Soares BC, Lima-Martins MV, Proietti FA (1999) HIV-(1/2) indeterminate western blot results: follow-up of asymptomatic blood donors in Belo Horizonte, Minas Gerais, Brazil. Our results indicate a lower proportion of HIV indeterminate WB results in southeastern China compared to previous reports, and the follow-up re-examination of patients with HIV indeterminate results should be performed. In addition, quantitative and qualitative HIV nucleic acid testing exhibited the highest sensitivity (96.3%) and specificity (97.85%), respectively. Among the Env, Gag, and Pol antibodies, samples with a Gag band showed the highest negative conversion rate (81.25%), whereas the highest positive conversion rate was observed in samples with an Env band (56.76%). The follow-up study revealed that the highest negative and positive conversion rates of HIV antibodies were in samples with a single p24 band (80.28%), and with gp160 and p24 bands (86.21%), respectively. The most common WB band patterns were p24, gp160 and p24, and gp160. The highest proportion of indeterminate results was observed in pregnant and lying-in women receiving physical examinations (16.67%), followed by that in voluntary blood donors (8.82%). In this study, 210 subjects with HIV-indeterminate WB results were detected from 6360 positive HIV screening samples between 20 in southeastern China, in which HIV-indeterminate WB results accounted for 3.30%. However, the effectiveness of these assays for the differential diagnosis of HIV-indeterminate WB samples remained undetermined. Nucleic acid detection has been used as a laboratory test for screening suspected or indeterminate samples. HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays, and the number of indeterminate samples may increase with the detection of HIV infections, which will present considerable challenges for the management of HIV/AIDS.













Western blot test for hiv